CHEK2 anticorps (pThr68)

N° du produit ABIN7464252

Cet anticorps Lapin Polyclonal détecte spécifiquement CHEK2 dans WB, IF et ICC. Il présente une réactivité envers Humain.

Aperçu rapide pour CHEK2 anticorps (pThr68) (ABIN7464252)

Antigène: CHEK2 (Checkpoint Kinase 2 (CHEK2))

Reactivité: Humain

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp CHEK2 est non-conjugé

Application: Western Blotting (WB), Immunofluorescence (IF), Immunocytochemistry (ICC)

Classe de qualité: KO Validated

Détail du produit anti-CHEK2 anticorps

Épitope: pThr68

Réactivité croisée: Humain

Purification: Purified by antigen-affinity chromatography.

Immunogène: Carrier-protein conjugated synthetic peptide surrounding phospho Thr68 of human Chk2. The exact sequence is proprietary.

Isotype: IgG

Information d'application

Indications d'application: WB: 1:500-1:3000. ICC/IF: 1:100-1:1000. Optimal dilutions/concentrations should be determined by the researcher. Not tested in other applications.

Commentaires:

Positive Control: 293T (100μM etoposide for 2hr) , HeLa

Validation: KO/KD, Orthogonal

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 0.73 mg/mL

Buffer: 1XPBS ( pH 7), 1 % BSA, 20 % Glycerol, 0.025 % ProClin 300

Agent conservateur: ProClin

Précaution d'utilisation: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: 4 °C,-20 °C

Stockage commentaire: Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4°C. For long-term storage, aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.

Détails sur CHEK2

Antigène: CHEK2 (Checkpoint Kinase 2 (CHEK2))

Autre désignation: checkpoint kinase 2

Sujet: Checkpoint kinase 2 , CDS1 , CHK2 , HuCds1 , LFS2 , PP1425 , RAD53 , hCds1,In response to DNA damage and replication blocks, cell cycle progression is halted through the control of critical cell cycle regulators. The protein encoded by this gene is a cell cycle checkpoint regulator and putative tumor suppressor. It contains a forkhead-associated protein interaction domain essential for activation in response to DNA damage and is rapidly phosphorylated in response to replication blocks and DNA damage. When activated, the encoded protein is known to inhibit CDC25C phosphatase, preventing entry into mitosis, and has been shown to stabilize the tumor suppressor protein p53, leading to cell cycle arrest in G1. In addition, this protein interacts with and phosphorylates BRCA1, allowing BRCA1 to restore survival after DNA damage. Mutations in this gene have been linked with Li-Fraumeni syndrome, a highly penetrant familial cancer phenotype usually associated with inherited mutations in TP53. Also, mutations in this gene are thought to confer a predisposition to sarcomas, breast cancer, and brain tumors. This nuclear protein is a member of the CDS1 subfamily of serine/threonine protein kinases. Three transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq]

Poids moléculaire: 61 kDa

ID gène: 11200

UniProt: O96017

Pathways: Signalisation p53, Apoptose, Cycle Cellulaire